2MWN

Talin-F3 / RIAM N-terminal Peptide complex

Summary for 2MWN

• Entry DOI: 10.2210/pdb2mwn/pdb
• NMR Information: BMRB: 25343
• Descriptor: Amyloid beta A4 precursor protein-binding family B member 1-interacting protein, Talin-1 (2 entities in total)
• Functional Keywords: riam, talin, integrin, structural protein-signaling protein complex, structural protein/signaling protein
• Biological source: Homo sapiens (human); More
• Cellular location: Cell membrane ; Peripheral membrane protein : Q7Z5R6; Cell projection, ruffle membrane ; Peripheral membrane protein ; Cytoplasmic side : Q9Y490
• Total number of polymer chains: 2
• Total formula weight: 13339.30

Authors

Yang, J.,Zhu, L.,Zhang, H.,Hirbawi, J.,Fukuda, K.,Dwivedi, P.,Liu, J.,Byzova, T.,Plow, E.F.,Wu, J.,Qin, J. (deposition date: 2014-11-13, release date: 2014-12-17, Last modification date: 2024-05-15)

Primary citation

Yang, J.,Zhu, L.,Zhang, H.,Hirbawi, J.,Fukuda, K.,Dwivedi, P.,Liu, J.,Byzova, T.,Plow, E.F.,Wu, J.,Qin, J.
Conformational activation of talin by RIAM triggers integrin-mediated cell adhesion.
Nat Commun, 5:5880-5880, 2014

PubMed Abstract: The membrane localization and activation of cytoskeletal protein talin are key steps to initiate the integrin transmembrane receptors' activation, which mediates many cellular adhesive responses such as cell migration, spreading and proliferation. RIAM, a membrane anchor and small GTPase RAP1 effector, is known to bind to the C-terminal rod domain of talin (talin-R) and promote localizations of talin to the membrane. Through systematic mapping analysis, we find that RIAM also binds to the N-terminal head of talin (talin-H), a crucial domain involved in binding and activating integrins. We show that the RIAM binding to talin-H sterically occludes the binding of a talin-R domain that otherwise masks the integrin-binding site on talin-H. We further provide functional evidence that such RIAM-mediated steric unmasking of talin triggers integrin activation. Our findings thus uncover a novel role for RIAM in conformational regulation of talin during integrin activation and cell adhesion.

PubMed: 25520155
DOI: 10.1038/ncomms6880

Experimental method

SOLUTION NMR