2MS6

Human Telomeric G-quadruplex DNA sequence (TTAGGGT)4 complexed with Flavonoid Quercetin

Summary for 2MS6

• Entry DOI: 10.2210/pdb2ms6/pdb
• Related: 2MS5
• NMR Information: BMRB: 25107
• Descriptor: DNA_(5'-D(*TP*TP*AP*GP*GP*GP*T)-3'), 3,5,7,3',4'-PENTAHYDROXYFLAVONE (2 entities in total)
• Functional Keywords: dna g-quadruplex, quercetin, telomeric sequence, dna
• Biological source: synthetic construct
• Total number of polymer chains: 4
• Total formula weight: 9278.25

Authors

Kumar, A.,Tawani, A. (deposition date: 2014-07-24, release date: 2015-01-28, Last modification date: 2024-05-01)

Primary citation

Tawani, A.,Kumar, A.
Structural Insight into the interaction of Flavonoids with Human Telomeric Sequence
Sci Rep, 5:17574-17574, 2015

PubMed Abstract: Flavonoids are a group of naturally available compounds that are an attractive source for drug discovery. Their potential to act as anti-tumourigenic and anti-proliferative agents has been reported previously but is not yet fully understood. Targeting human telomeric G-quadruplex DNA could be one of the mechanisms by which these flavonoids exert anticancer activity. We have performed detailed biophysical studies for the interaction of four representative flavonoids, Luteolin, Quercetin, Rutin and Genistein, with the human telomeric G-quadruplex sequence tetramolecular d-(T2AG3T) (Tel7). In addition, we used NMR spectroscopy to derive the first model for the complex formed between Quercetin and G-quadruplex sequence. The model showed that Quercetin stabilises the G-quadruplex structure and does not open the G-tetrad. It interacts with the telomeric sequence through π-stacking at two sites: between T1pT2 and between G6pT7. Based on our findings, we suggest that Quercetin could be a potent candidate for targeting the telomere and thus, act as a potent anti-cancer agent.

PubMed: 26627543
DOI: 10.1038/srep17574

Experimental method

SOLUTION NMR