2MP1

Solution structure of the human chemokine CCL19

2MP1 の概要

• エントリーDOI: 10.2210/pdb2mp1/pdb
• NMR情報: BMRB: 19960
• 分子名称: C-C motif chemokine 19 (1 entity in total)
• 機能のキーワード: chemokines, chemokine receptors, ccl19, ccl21, ccr7, signaling protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: Q99731
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8814.28

構造登録者

Veldkamp, C.T.,Peterson, F.C.,Gabel-Eissens, S.J.,Gillitzer, M.L. (登録日: 2014-05-09, 公開日: 2015-06-10, 最終更新日: 2024-10-30)

主引用文献

Veldkamp, C.T.,Kiermaier, E.,Gabel-Eissens, S.J.,Gillitzer, M.L.,Lippner, D.R.,DiSilvio, F.A.,Mueller, C.J.,Wantuch, P.L.,Chaffee, G.R.,Famiglietti, M.W.,Zgoba, D.M.,Bailey, A.A.,Bah, Y.,Engebretson, S.J.,Graupner, D.R.,Lackner, E.R.,LaRosa, V.D.,Medeiros, T.,Olson, M.L.,Phillips, A.J.,Pyles, H.,Richard, A.M.,Schoeller, S.J.,Touzeau, B.,Williams, L.G.,Sixt, M.,Peterson, F.C.
Solution Structure of CCL19 and Identification of Overlapping CCR7 and PSGL-1 Binding Sites.
Biochemistry, 54:4163-4166, 2015

PubMed Abstract: CCL19 and CCL21 are chemokines involved in the trafficking of immune cells, particularly within the lymphatic system, through activation of CCR7. Concurrent expression of PSGL-1 and CCR7 in naive T-cells enhances recruitment of these cells to secondary lymphoid organs by CCL19 and CCL21. Here the solution structure of CCL19 is reported. It contains a canonical chemokine domain. Chemical shift mapping shows the N-termini of PSGL-1 and CCR7 have overlapping binding sites for CCL19 and binding is competitive. Implications for the mechanism of PSGL-1's enhancement of resting T-cell recruitment are discussed.

PubMed: 26115234
DOI: 10.1021/acs.biochem.5b00560

実験手法

SOLUTION NMR