2MOZ

Structure of the Membrane Protein MerF, a Bacterial Mercury Transporter, Improved by the Inclusion of Chemical Shift Anisotropy Constraints

Summary for 2MOZ

Entry DOI	10.2210/pdb2moz/pdb
Related	2M67
NMR Information	BMRB: 19115
Descriptor	MerF (1 entity in total)
Functional Keywords	membrane protein, membrane protein structures by solution nmr, mpsbynmr, psi-biology, structural genomics
Biological source	Morganella morganii
Total number of polymer chains	1
Total formula weight	8670.40
Authors	Tian, Y.,Lu, G.J.,Marassi, F.M.,Opella, S.J.,Membrane Protein Structures by Solution NMR (MPSbyNMR) (deposition date: 2014-05-07, release date: 2014-07-30, Last modification date: 2024-05-15)
Primary citation	Tian, Y.,Lu, G.J.,Marassi, F.M.,Opella, S.J. Structure of the membrane protein MerF, a bacterial mercury transporter, improved by the inclusion of chemical shift anisotropy constraints. J.Biomol.Nmr, 60:67-71, 2014 Cited by PubMed Abstract: MerF is a mercury transport membrane protein from the bacterial mercury detoxification system. By performing a solid-state INEPT experiment and measuring chemical shift anisotropy frequencies in aligned samples, we are able to improve on the accuracy and precision of the initial structure that we presented. MerF has four N-terminal and eleven C-terminal residues that are mobile and unstructured in phospholipid bilayers. The structure presented here has average pairwise RMSDs of 1.78 Å for heavy atoms and 0.92 Å for backbone atoms. PubMed: 25103921 DOI: 10.1007/s10858-014-9852-0 PDB entries with the same primary citation
Experimental method	SOLUTION NMR

Structure validation