2MKV
Structure of the NA,K-ATPASE regulatory protein FXYD2b in micelles
Summary for 2MKV
| Entry DOI | 10.2210/pdb2mkv/pdb |
| Related | 2JO1 2JP3 |
| NMR Information | BMRB: 19797 |
| Descriptor | Sodium/potassium-transporting ATPase subunit gamma (1 entity in total) |
| Functional Keywords | fxyd2b, na, k-atpase, fxyd, micelle, transport protein |
| Biological source | Homo sapiens (human) |
| Cellular location | Membrane ; Single-pass type III membrane protein : P54710 |
| Total number of polymer chains | 1 |
| Total formula weight | 7331.31 |
| Authors | Marassi, F.M.,Gong, X. (deposition date: 2014-02-13, release date: 2014-05-14, Last modification date: 2024-05-15) |
| Primary citation | Gong, X.M.,Ding, Y.,Yu, J.,Yao, Y.,Marassi, F.M. Structure of the Na,K-ATPase regulatory protein FXYD2b in micelles: Implications for membrane-water interfacial arginines. Biochim.Biophys.Acta, 1848:299-306, 2015 Cited by PubMed Abstract: FXYD2 is a membrane protein responsible for regulating the function of the Na,K-ATPase in mammalian kidney epithelial cells. Here we report the structure of FXYD2b, one of two splice variants of the protein, determined by NMR spectroscopy in detergent micelles. Solid-state NMR characterization of the protein embedded in phospholipid bilayers indicates that several arginine side chains may be involved in hydrogen bond interactions with the phospholipid polar head groups. The structure and the NMR data suggest that FXYD2b could regulate the Na,K-ATPase by modulating the effective membrane surface electrostatics near the ion binding sites of the pump. PubMed: 24794573DOI: 10.1016/j.bbamem.2014.04.021 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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