2MJY

Solution structure of synthetic Mamba-1 peptide

Summary for 2MJY

• Entry DOI: 10.2210/pdb2mjy/pdb
• NMR Information: BMRB: 19746
• Descriptor: Mambalgin-1 (1 entity in total)
• Functional Keywords: toxin, manba
• Biological source: Dendroaspis polylepis polylepis (Black mamba)
• Cellular location: Secreted : P0DKR6
• Total number of polymer chains: 1
• Total formula weight: 6574.64

Authors

He, Y.,Wu, F.,Tian, C. (deposition date: 2014-01-21, release date: 2015-01-28, Last modification date: 2024-11-06)

Primary citation

Pan, M.,He, Y.,Wen, M.,Wu, F.,Sun, D.,Li, S.,Zhang, L.,Li, Y.,Tian, C.
One-pot hydrazide-based native chemical ligation for efficient chemical synthesis and structure determination of toxin Mambalgin-1
Chem.Commun.(Camb.), 50:5837-5839, 2014

PubMed Abstract: An efficient one-pot chemical synthesis of snake venom toxin Mambalgin-1 was achieved using an azide-switch strategy combined with hydrazide-based native chemical ligation. Synthetic Mambalgin-1 exhibited a well-defined structure after sequential folding in vitro. NMR spectroscopy revealed a three-finger toxin family structure, and the synthetic toxin inhibited human acid-sensing ion channel 1a.

PubMed: 24619065
DOI: 10.1039/c4cc00779d

Experimental method

SOLUTION NMR