2MJJ

A tetrahelical DNA fold adopted by alternating GGG and GCG tracts

Summary for 2MJJ

• Entry DOI: 10.2210/pdb2mjj/pdb
• NMR Information: BMRB: 19728
• Descriptor: 5'-D(*GP*GP*GP*AP*GP*CP*GP*AP*GP*GP*GP*AP*GP*CP*G)-3' (1 entity in total)
• Functional Keywords: tandem repeat, dna
• Total number of polymer chains: 2
• Total formula weight: 9530.17

Authors

Kocman, V.,Plavec, J. (deposition date: 2014-01-10, release date: 2014-12-24, Last modification date: 2024-05-15)

Primary citation

Kocman, V.,Plavec, J.
A tetrahelical DNA fold adopted by tandem repeats of alternating GGG and GCG tracts.
Nat Commun, 5:5831-5831, 2014

PubMed Abstract: DNA can form diverse higher-order structures, whose details are greatly dependent on nucleotide sequence. G-rich sequences containing four or more repeats of three guanines are expected to form G-quadruplexes. Here we show that DNA sequences with GGGAGCG repeats found in the regulatory region of the PLEKHG3 gene are capable of forming tetrahelical DNA structures that are distinct from G-quadruplexes. The d(GGGAGCGAGGGAGCG) sequence, VK1, forms a dimer. Two VK1 sequences connected by an adenine residue, VK2, fold into a monomer, which shares identical structural characteristics with the VK1 fold. Their four-stranded architectures are stabilized by four G-C, four G-A and six G-G base pairs. No G-quartets or Hoogsteen-type hydrogen-bonded guanine residues are present and the overall topology is conserved in the presence of Li(+), Na(+), K(+) and NH4(+) ions. Unique structural features include two edgewise loops on each side of the structure stabilized by three G-G base pairs in N1-carbonyl symmetric geometry.

PubMed: 25500730
DOI: 10.1038/ncomms6831

Experimental method

SOLUTION NMR