2MI8

Solution structure of lysine-free (K0) ubiquitin

Summary for 2MI8

• Entry DOI: 10.2210/pdb2mi8/pdb
• NMR Information: BMRB: 19670
• Descriptor: Ubiquitin (1 entity in total)
• Functional Keywords: signaling protein
• Biological source: Homo sapiens (human)
• Cellular location: Ubiquitin: Cytoplasm (By similarity): P0CG48
• Total number of polymer chains: 1
• Total formula weight: 8772.93

Authors

Huang, T.,Li, J.R.,Byrd, A.R. (deposition date: 2013-12-09, release date: 2014-04-02, Last modification date: 2024-05-15)

Primary citation

Huang, T.,Li, J.,Byrd, R.A.
Solution structure of lysine-free (K0) ubiquitin.
Protein Sci., 23:662-667, 2014

PubMed Abstract: Lysine-free ubiquitin (K0-Ub) is commonly used to study the ubiquitin-signaling pathway, where it is assumed to have the same structure and function as wild-type ubiquitin (wt-Ub). However, the K0-Ub (15) N heteronuclear single quantum correlation NMR spectrum differs significantly from wt-Ub and the melting temperature is depressed by 19°C, raising the question of the structural integrity and equivalence to wt-Ub. The three-dimensional structure of K0-Ub was determined by solution NMR, using chemical shift and residual dipolar coupling data. K0-Ub adopts the same backbone structure as wt-Ub, and all significant chemical shifts can be related to interactions impacted by the K to R mutations.

PubMed: 24591328
DOI: 10.1002/pro.2450

Experimental method

SOLUTION NMR