2MHF

Solution structure of the cyclic-nucleotide binding homology domain of a KCNH channel

Summary for 2MHF

• Entry DOI: 10.2210/pdb2mhf/pdb
• NMR Information: BMRB: 19621
• Descriptor: Uncharacterized protein (1 entity in total)
• Functional Keywords: kcnh, dynamics, ion channel, nucleotide binding domain, transport protein
• Biological source: Danio rerio (leopard danio, zebra danio, zebra fish)
• Total number of polymer chains: 1
• Total formula weight: 16182.66

Authors

Li, Q.,Ng, H. (deposition date: 2013-11-21, release date: 2014-04-02, Last modification date: 2024-05-15)

Primary citation

Li, Q.,Ng, H.Q.,Yoon, H.S.,Kang, C.
Solution structure of the cyclic-nucleotide binding homology domain of a KCNH channel.
J.Struct.Biol., 186:68-74, 2014

PubMed Abstract: The carboxy-terminal region of the KCNH family of potassium channels contains a cyclic-nucleotide binding homology domain (CNBHD) that is important for channel gating and trafficking. The solution structure of the CNBHD of the KCNH potassium of zebrafish was determined using solution NMR spectroscopy. This domain exists as a monomer under solution conditions and adopts a similar fold to that determined by X-ray crystallography. The CNBHD does not bind cAMP because residue Y740 blocks the entry of cyclic-nucleotide to the binding pocket. Relaxation results show that the CNBHD is rigid except that some residues in the loop between β6 and β7 are flexible. Our results will be useful to understand the gating mechanism of KCNH family members through the CNBHD.

PubMed: 24632450
DOI: 10.1016/j.jsb.2014.03.008

Experimental method

SOLUTION NMR