2MES

Backbone 1H, 13C, 15N resonance assignments of calcium-bound calmodulin in complex with PSD95 N-terminal peptide

Summary for 2MES

• Entry DOI: 10.2210/pdb2mes/pdb
• NMR Information: BMRB: 19238
• Descriptor: Calmodulin, Disks large homolog 4, CALCIUM ION (3 entities in total)
• Functional Keywords: protein/peptide, metal binding protein
• Biological source: Xenopus laevis (clawed frog,common platanna,platanna); More
• Cellular location: Cell membrane ; Peripheral membrane protein : P78352
• Total number of polymer chains: 2
• Total formula weight: 24880.45

Authors

Zhang, Y.,Ames, J.B. (deposition date: 2013-09-26, release date: 2014-12-24, Last modification date: 2024-05-15)

Primary citation

Zhang, Y.,Matt, L.,Patriarchi, T.,Malik, Z.A.,Chowdhury, D.,Park, D.K.,Renieri, A.,Ames, J.B.,Hell, J.W.
Capping of the N-terminus of PSD-95 by calmodulin triggers its postsynaptic release.
Embo J., 33:1341-1353, 2014

PubMed Abstract: Postsynaptic density protein-95 (PSD-95) is a central element of the postsynaptic architecture of glutamatergic synapses. PSD-95 mediates postsynaptic localization of AMPA receptors and NMDA receptors and plays an important role in synaptic plasticity. PSD-95 is released from postsynaptic membranes in response to Ca(2+) influx via NMDA receptors. Here, we show that Ca(2+)/calmodulin (CaM) binds at the N-terminus of PSD-95. Our NMR structure reveals that both lobes of CaM collapse onto a helical structure of PSD-95 formed at its N-terminus (residues 1-16). This N-terminal capping of PSD-95 by CaM blocks palmitoylation of C3 and C5, which is required for postsynaptic PSD-95 targeting and the binding of CDKL5, a kinase important for synapse stability. CaM forms extensive hydrophobic contacts with Y12 of PSD-95. The PSD-95 mutant Y12E strongly impairs binding to CaM and Ca(2+)-induced release of PSD-95 from the postsynaptic membrane in dendritic spines. Our data indicate that CaM binding to PSD-95 serves to block palmitoylation of PSD-95, which in turn promotes Ca(2+)-induced dissociation of PSD-95 from the postsynaptic membrane.

PubMed: 24705785
DOI: 10.1002/embj.201488126

Experimental method

SOLUTION NMR