2MB2
parallel-stranded G-quadruplex in DNA poly-G stretches
Summary for 2MB2
| Entry DOI | 10.2210/pdb2mb2/pdb |
| NMR Information | BMRB: 19386 |
| Descriptor | DNA_(5'-D(*TP*TP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*T)-3') (1 entity in total) |
| Functional Keywords | g-tracts, g-quadruplex, propeller structure, poly-g stretches, dna |
| Total number of polymer chains | 1 |
| Total formula weight | 5805.71 |
| Authors | Sengar, A.,Heddi, B.,Phan, A.T. (deposition date: 2013-07-24, release date: 2014-12-03, Last modification date: 2024-05-15) |
| Primary citation | Sengar, A.,Heddi, B.,Phan, A.T. Formation of g-quadruplexes in poly-g sequences: structure of a propeller-type parallel-stranded g-quadruplex formed by a g15 stretch. Biochemistry, 53:7718-7723, 2014 Cited by PubMed Abstract: Poly-G sequences are found in different genomes including human and have the potential to form higher-order structures with various applications. Previously, long poly-G sequences were thought to lead to multiple possible ways of G-quadruplex folding, rendering their structural characterization challenging. Here we investigate the structure of G-quadruplexes formed by poly-G sequences d(TTG(n)T), where n = 12 to 19. Our data show the presence of multiple and/or higher-order G-quadruplex structures in most sequences. Strikingly, NMR spectra of the TTG₁₅T sequence containing a stretch of 15 continuous guanines are exceptionally well-resolved and indicate the formation of a well-defined G-quadruplex structure. The NMR solution structure of this sequence revealed a propeller-type parallel-stranded G-quadruplex containing three G-tetrad layers and three single-guanine propeller loops. The same structure can potentially form anywhere along a long G(n) stretch, making it unique for molecular recognition by other cellular molecules. PubMed: 25375976DOI: 10.1021/bi500990v PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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