2M9J
NMR solution structure of Pin1 WW domain mutant 6-1g
Summary for 2M9J
Entry DOI | 10.2210/pdb2m9j/pdb |
Related | 2M9E 2M9F 2M9I |
NMR Information | BMRB: 19296 |
Descriptor | Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, 2-acetamido-2-deoxy-beta-D-glucopyranose (2 entities in total) |
Functional Keywords | n-glycosylation, enhanced aromatic sequon, ww domain, ch-pi interaction, isomerase |
Biological source | Homo sapiens (human) |
Total number of polymer chains | 1 |
Total formula weight | 4243.72 |
Authors | Enck, S.,Chen, W.,Price, J.L.,Powers, E.T.,Wong, C.,Dyson, H.J.,Kelly, J.W. (deposition date: 2013-06-10, release date: 2013-06-26, Last modification date: 2020-07-29) |
Primary citation | Chen, W.,Enck, S.,Price, J.L.,Powers, D.L.,Powers, E.T.,Wong, C.H.,Dyson, H.J.,Kelly, J.W. Structural and energetic basis of carbohydrate-aromatic packing interactions in proteins. J.Am.Chem.Soc., 135:9877-9884, 2013 Cited by PubMed Abstract: Carbohydrate-aromatic interactions mediate many biological processes. However, the structure-energy relationships underpinning direct carbohydrate-aromatic packing interactions in aqueous solution have been difficult to assess experimentally and remain elusive. Here, we determine the structures and folding energetics of chemically synthesized glycoproteins to quantify the contributions of the hydrophobic effect and CH-π interactions to carbohydrate-aromatic packing interactions in proteins. We find that the hydrophobic effect contributes significantly to protein-carbohydrate interactions. Interactions between carbohydrates and aromatic amino acid side chains, however, are supplemented by CH-π interactions. The strengths of experimentally determined carbohydrate CH-π interactions do not correlate with the electrostatic properties of the involved aromatic residues, suggesting that the electrostatic component of CH-π interactions in aqueous solution is small. Thus, tight binding of carbohydrates and aromatic residues is driven by the hydrophobic effect and CH-π interactions featuring a dominating dispersive component. PubMed: 23742246DOI: 10.1021/ja4040472 PDB entries with the same primary citation |
Experimental method | SOLUTION NMR |
Structure validation
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