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2M6P

The actinobacterial transcription factor RbpA binds to the principal sigma subunit of RNA polymerase

Summary for 2M6P
Entry DOI10.2210/pdb2m6p/pdb
Related2M6O
NMR InformationBMRB: 19149
Descriptoruncharacterized protein Mb2076 (1 entity in total)
Functional Keywordsmv2050, mtb, rnap, sigma factor, transcription
Biological sourceMycobacterium bovis
Total number of polymer chains1
Total formula weight5213.68
Authors
Liu, B.,Parsy, M.,Paget, M.,Matthews, S. (deposition date: 2013-04-06, release date: 2013-05-08, Last modification date: 2024-05-01)
Primary citationTabib-Salazar, A.,Liu, B.,Doughty, P.,Lewis, R.A.,Ghosh, S.,Parsy, M.L.,Simpson, P.J.,O'Dwyer, K.,Matthews, S.J.,Paget, M.S.
The actinobacterial transcription factor RbpA binds to the principal sigma subunit of RNA polymerase.
Nucleic Acids Res., 41:5679-5691, 2013
Cited by
PubMed Abstract: RbpA is a small non-DNA-binding transcription factor that associates with RNA polymerase holoenzyme and stimulates transcription in actinobacteria, including Streptomyces coelicolor and Mycobacterium tuberculosis. RbpA seems to show specificity for the vegetative form of RNA polymerase as opposed to alternative forms of the enzyme. Here, we explain the basis of this specificity by showing that RbpA binds directly to the principal σ subunit in these organisms, but not to more diverged alternative σ factors. Nuclear magnetic resonance spectroscopy revealed that, although differing in their requirement for structural zinc, the RbpA orthologues from S. coelicolor and M. tuberculosis share a common structural core domain, with extensive, apparently disordered, N- and C-terminal regions. The RbpA-σ interaction is mediated by the C-terminal region of RbpA and σ domain 2, and S. coelicolor RbpA mutants that are defective in binding σ are unable to stimulate transcription in vitro and are inactive in vivo. Given that RbpA is essential in M. tuberculosis and critical for growth in S. coelicolor, these data support a model in which RbpA plays a key role in the σ cycle in actinobacteria.
PubMed: 23605043
DOI: 10.1093/nar/gkt277
PDB entries with the same primary citation
Experimental method
SOLUTION NMR
Structure validation

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