2M5M

Atomic-resolution structure of a triplet cross-beta amyloid fibril

2M5M の概要

• エントリーDOI: 10.2210/pdb2m5m/pdb
• 関連するPDBエントリー: 2M5K 2M5N 3ZPK
• EMDBエントリー: 2323 2324 5590
• NMR情報: BMRB: 19060
• 分子名称: Transthyretin (1 entity in total)
• 機能のキーワード: amyloid fibril, cross-beta structure, protein fibril
• 由来する生物種: Rattus norvegicus (rat)
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 14380.39

構造登録者

Fitzpatrick, A.W.P.,Debelouchina, G.T.,Bayro, M.J.,Clare, D.K.,Caporini, M.A.,Bajaj, V.S.,Jaroniec, C.P.,Wang, L.,Ladizhansky, V.,Muller, S.,MacPhee, C.E.,Waudby, C.A.,Mott, H.R.,de Simone, A.,Knowles, T.P.J.,Saibil, H.R.,Vendruscolo, M.,Orlova, E.V.,Griffin, R.G.,Dobson, C.M. (登録日: 2013-02-27, 公開日: 2013-12-04, 最終更新日: 2024-05-15)

主引用文献

Fitzpatrick, A.W.,Debelouchina, G.T.,Bayro, M.J.,Clare, D.K.,Caporini, M.A.,Bajaj, V.S.,Jaroniec, C.P.,Wang, L.,Ladizhansky, V.,Muller, S.A.,MacPhee, C.E.,Waudby, C.A.,Mott, H.R.,De Simone, A.,Knowles, T.P.,Saibil, H.R.,Vendruscolo, M.,Orlova, E.V.,Griffin, R.G.,Dobson, C.M.
Atomic structure and hierarchical assembly of a cross-beta amyloid fibril.
Proc.Natl.Acad.Sci.USA, 110:5468-5473, 2013

PubMed Abstract: The cross-β amyloid form of peptides and proteins represents an archetypal and widely accessible structure consisting of ordered arrays of β-sheet filaments. These complex aggregates have remarkable chemical and physical properties, and the conversion of normally soluble functional forms of proteins into amyloid structures is linked to many debilitating human diseases, including several common forms of age-related dementia. Despite their importance, however, cross-β amyloid fibrils have proved to be recalcitrant to detailed structural analysis. By combining structural constraints from a series of experimental techniques spanning five orders of magnitude in length scale--including magic angle spinning nuclear magnetic resonance spectroscopy, X-ray fiber diffraction, cryoelectron microscopy, scanning transmission electron microscopy, and atomic force microscopy--we report the atomic-resolution (0.5 Å) structures of three amyloid polymorphs formed by an 11-residue peptide. These structures reveal the details of the packing interactions by which the constituent β-strands are assembled hierarchically into protofilaments, filaments, and mature fibrils.

PubMed: 23513222
DOI: 10.1073/pnas.1219476110

実験手法

ELECTRON MICROSCOPY (12.2 Å)
SOLID-STATE NMR (12.2 Å)