2M3S
Calmodulin, i85l, f92e, h107i, l112r, a128t, m144r mutant
Summary for 2M3S
| Entry DOI | 10.2210/pdb2m3s/pdb |
| Related | 2KZ2 |
| NMR Information | BMRB: 18976 |
| Descriptor | Calmodulin, CALCIUM ION (2 entities in total) |
| Functional Keywords | calmodulin, metal binding protein |
| Biological source | Gallus gallus (chicken) |
| Total number of polymer chains | 1 |
| Total formula weight | 17270.10 |
| Authors | Moroz, Y.S.,Wu, Y.,Cheng, H.,Roder, H.,Korendovych, I.V. (deposition date: 2013-01-25, release date: 2013-07-24, Last modification date: 2024-05-15) |
| Primary citation | Moroz, O.V.,Moroz, Y.S.,Wu, Y.,Olsen, A.B.,Cheng, H.,Mack, K.L.,McLaughlin, J.M.,Raymond, E.A.,Zhezherya, K.,Roder, H.,Korendovych, I.V. A single mutation in a regulatory protein produces evolvable allosterically regulated catalyst of nonnatural reaction. Angew.Chem.Int.Ed.Engl., 52:6246-6249, 2013 Cited by PubMed Abstract: a strategically placed single mutation in a non-enzymatic protein scaffold produced AlleyCat, a small, allosterically regulated catalyst of Kemp elimination. In only 7 rounds of directed evolution enzymatic efficiency of the original 74 amino acid residue catalyst was improved more than 220-fold to achieve k value higher than that of catalytic antibodies for the same reaction, still preserving allosteric regulation. PubMed: 23630096DOI: 10.1002/anie.201302339 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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