2M1K

Interaction of Human S100A6 (C3S) with V domain of Receptor for Advanced Glycation End products (RAGE)

2M1K の概要

• エントリーDOI: 10.2210/pdb2m1k/pdb
• 関連するPDBエントリー: 1K9K 2E5E
• NMR情報: BMRB: 18868
• 分子名称: Protein S100-A6, Advanced glycosylation end product-specific receptor (2 entities in total)
• 機能のキーワード: s100a6 c3s, rage v, heterotetrameric, haddock model, protein binding
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus envelope: P06703; Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. Isoform 10: Cell membrane ; Single-pass type I membrane protein : Q15109
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 42789.34

構造登録者

Gupta, A.A.,Yu, C. (登録日: 2012-11-29, 公開日: 2015-03-25, 最終更新日: 2024-11-06)

主引用文献

Mohan, S.K.,Gupta, A.A.,Yu, C.
Interaction of the S100A6 mutant (C3S) with the V domain of the receptor for advanced glycation end products (RAGE).
Biochem.Biophys.Res.Commun., 434:328-333, 2013

PubMed Abstract: S100A6 is involved in several vital biological functions, such as calcium sensing and cell proliferation. It is a homodimeric protein that belongs to the S100 protein family. The receptor for advanced glycation end products (RAGE) has been shown to play a role in the progression of various disease conditions, such as diabetes and immune/inflammatory disorders. Information regarding the association of RAGE with S100 proteins at a molecular level is useful to understand the diversity of the RAGE signaling pathways. In this report, biomolecular NMR techniques were utilized for the resonance assignment of the C3S mutation in human S100A6 and characterizing its interaction with the RAGE V domain. Further binding affinity between S100A6m and the RAGE V domain was determined by isothermal titration calorimetric studies. HADDOCK was used to generate a heterotetramer model of the S100A6m-RAGE V domain complex. This model provides an important insights into the S100-RAGE cellular signaling pathway.

PubMed: 23537648
DOI: 10.1016/j.bbrc.2013.03.049

実験手法

SOLUTION NMR