2LXV

Dimeric pil-e g-quadruplex dna from neisseria gonorrhoeae, NMR 11 structures

Summary for 2LXV

• Entry DOI: 10.2210/pdb2lxv/pdb
• NMR Information: BMRB: 18699
• Descriptor: 5'-D(*GP*GP*GP*TP*GP*GP*GP*TP*TP*GP*GP*GP*TP*GP*GP (1 entity in total)
• Functional Keywords: g-tetrad, all-parallel quadruplex, 5'-end stacked dimer, pilin antige, dna
• Total number of polymer chains: 2
• Total formula weight: 10244.57

Authors

Kuryavyi, V.,Patel, D.J. (deposition date: 2012-08-31, release date: 2012-11-28, Last modification date: 2024-05-15)

Primary citation

Kuryavyi, V.,Cahoon, L.A.,Seifert, H.S.,Patel, D.J.
RecA-Binding pilE G4 Sequence Essential for Pilin Antigenic Variation Forms Monomeric and 5' End-Stacked Dimeric Parallel G-Quadruplexes.
Structure, 20:2090-2102, 2012

PubMed Abstract: Neisseria gonorrhoeae is an obligate human pathogen that can escape immune surveillance through antigenic variation of surface structures such as pili. A G-quadruplex-forming (G4) sequence (5'-G(3)TG(3)TTG(3)TG(3)) located upstream of the N. gonorrhoeae pilin expression locus (pilE) is necessary for initiation of pilin antigenic variation, a recombination-based, high-frequency, diversity-generation system. We have determined NMR-based structures of the all parallel-stranded monomeric and 5' end-stacked dimeric pilE G-quadruplexes in monovalent cation-containing solutions. We demonstrate that the three-layered all parallel-stranded monomeric pilE G-quadruplex containing single-residue double-chain reversal loops, which can be modeled without steric clashes into the 3 nt DNA-binding site of RecA, binds and promotes E. coli RecA-mediated strand exchange in vitro. We discuss how interactions between RecA and monomeric pilE G-quadruplex could facilitate the specialized recombination reactions leading to pilin diversification.

PubMed: 23085077
DOI: 10.1016/j.str.2012.09.013

Experimental method

SOLUTION NMR