2LPW
human CEB25 minisatellite G-quadruplex
Summary for 2LPW
| Entry DOI | 10.2210/pdb2lpw/pdb |
| Related | 2KYO |
| NMR Information | BMRB: 18279 |
| Descriptor | DNA (26-MER) (1 entity in total) |
| Functional Keywords | g-quadruplex, dna |
| Total number of polymer chains | 1 |
| Total formula weight | 8275.31 |
| Authors | Amrane, S.,Adrian, M.,Heddi, B.,Serero, A.,Nicolas, A.,Mergny, J.L.,Phan, A.T. (deposition date: 2012-02-20, release date: 2012-03-07, Last modification date: 2024-05-15) |
| Primary citation | Amrane, S.,Adrian, M.,Heddi, B.,Serero, A.,Nicolas, A.,Mergny, J.L.,Phan, A.T. Formation of Pearl-Necklace Monomorphic G-Quadruplexes in the Human CEB25 Minisatellite. J.Am.Chem.Soc., 134:5807-5816, 2012 Cited by PubMed Abstract: CEB25 is a human minisatellite locus, composed of slightly polymorphic 52-nucleotide (nt) tandem repeats. Genetically, most if not all individuals of the human population are heterozygous, carrying alleles ranging from 0.5 to 20 kb, maintained by mendelian inheritance but also subject to germline instability. To provide insights on the biological role of CEB25, we interrogated its structural features. We report the NMR structure of the G-quadruplex formed by the conserved 26-nt G-rich fragment of the CEB25 motif. In K(+) solution, this sequence forms a propeller-type parallel-stranded G-quadruplex involving a 9-nt central double-chain-reversal loop. This long loop is anchored to the 5'-end of the sequence by an A·T Watson-Crick base pair and a potential G·A noncanonical base pair. These base pairs contribute to the stability of the overall G-quadruplexstructure, as measured by an increase of about 17 kcal/mol in enthalpy or 6 °C in melting temperature. Further, we demonstrate that such a monomorphic structure is formed within longer sequence contexts folding into a pearl-necklace structure. PubMed: 22376028DOI: 10.1021/ja208993r PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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