2LP8

SOLUTION STRUCTURE OF AN APOPTOSIS ACTIVATING PHOTOSWITCHABLE BAK PEPTIDE BOUND to BCL-XL

2LP8 の概要

• エントリーDOI: 10.2210/pdb2lp8/pdb
• 関連するPDBエントリー: 2LPC
• NMR情報: BMRB: 18238
• 分子名称: Bcl-2-like protein 1, Bcl-2 homologous antagonist/killer, 3,3'-(E)-diazene-1,2-diylbis{6-[(chloroacetyl)amino]benzenesulfonic acid} (3 entities in total)
• 機能のキーワード: apoptosis, azobenzene, photoswitch, photocontrol, apoptosis inhibitor, apoptosis-apoptosis activator complex, apoptosis/apoptosis activator
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Mitochondrion membrane; Single-pass membrane protein: Q07817; Mitochondrion membrane; Single-pass membrane protein (Potential): Q16611
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 23629.80

構造登録者

Wysoczanski, P.,Mart, R.J.,Loveridge, J.E.,Williams, C.,Whittaker, S.B.-M.,Crump, M.P.,Allemann, R.K. (登録日: 2012-02-03, 公開日: 2012-04-18, 最終更新日: 2021-08-18)

主引用文献

Wysoczanski, P.,Mart, R.J.,Loveridge, E.J.,Williams, C.,Whittaker, S.B.,Crump, M.P.,Allemann, R.K.
NMR Solution Structure of a Photoswitchable Apoptosis Activating Bak Peptide Bound to Bcl-x(L).
J.Am.Chem.Soc., 134:7644-7647, 2012

PubMed Abstract: The Bcl-2 family of proteins includes the major regulators and effectors of the intrinsic apoptosis pathway. Cancers are frequently formed when activation of the apoptosis mechanism is compromised either by misregulated expression of prosurvival family members or, more frequently, by damage to the regulatory pathways that trigger intrinsic apoptosis. Short peptides derived from the pro-apoptotic members of the Bcl-2 family can activate mechanisms that ultimately lead to cell death. The recent development of photocontrolled peptides that are able to change their conformation and activity upon irradiation with an external light source has provided new tools to target cells for apoptosis induction with temporal and spatial control. Here, we report the first NMR solution structure of a photoswitchable peptide derived from the proapoptotic protein Bak in complex with the antiapoptotic protein Bcl-x(L). This structure provides insight into the molecular mechanism, by which the increased affinity of such photopeptides compared to their native forms is achieved, and offers a rationale for the large differences in the binding affinities between the helical and nonhelical states.

PubMed: 22515821
DOI: 10.1021/ja302390a

実験手法

SOLUTION NMR