2LM9
The NMR structure of a major allergen from dust mite
Summary for 2LM9
| Entry DOI | 10.2210/pdb2lm9/pdb |
| NMR Information | BMRB: 18107 |
| Descriptor | Blo t 21 allergen (1 entity in total) |
| Functional Keywords | dust mite, allergen |
| Biological source | Blomia tropicalis (Mite) |
| Total number of polymer chains | 1 |
| Total formula weight | 11208.64 |
| Authors | |
| Primary citation | Tan, K.W.,Ong, T.C.,Gao, Y.F.,Tiong, Y.S.,Wong, K.N.,Chew, F.T.,Mok, Y.K. NMR structure and IgE epitopes of Blo t 21, a major dust mite allergen from Blomia tropicalis J.Biol.Chem., 2012 Cited by PubMed Abstract: Blo t 21 is a paralogue of the group 5 allergen, Blo t 5, a major allergen from the dust mite Blomia tropicalis. Blo t 21 has moderate sequence identity (40.7%) to Blo t 5 and low to moderate cross-reactivity to Blo t 5. In B. tropicalis, the most prevalent and allergenic allergens are in the order of Blo t 21, Blo t 5, and Blo t 7. Here, we determined the NMR solution structure of Blo t 21, which represents the first structure of the group 21 dust mite allergen. The structure of Blo t 21 closely resembles the structures of Blo t 5 and Der p 5, comprising three anti-parallel α-helices arranged in a helical bundle. Using site-directed mutagenesis and specific IgE binding ELISA, Blo t 21 was found to contain both conserved and unique charged IgE epitope residues at the L2 loop region and on helix α3. Cross-inhibition assays confirmed that Blo t 21 has a low to moderate cross-reactivity with Blo t 5 and Der p 5 and represents a novel group of major allergen in B. tropicalis. In addition to group 5 allergens, Blo t 21 has also a low to moderate cross-reactivity with group 21 allergens from Dermatophagoides mites, confirming that B. tropicalis is a major and distinct source of dust mite allergens. PubMed: 22887997DOI: 10.1074/jbc.M112.348730 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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