2LK7
Monomer-dimer equilibrium for 5'-5' stacking of propeller-type parallel-stranded G-quadruplexes: NMR structural study
Summary for 2LK7
| Entry DOI | 10.2210/pdb2lk7/pdb |
| NMR Information | BMRB: 17980 |
| Descriptor | DNA (5'-D(*TP*TP*GP*GP*GP*TP*GP*GP*GP*TP*GP*GP*GP*TP*GP*GP*GP*T)-3') (1 entity in total) |
| Functional Keywords | dna, g-quadruplex, propeller type, monomer |
| Total number of polymer chains | 1 |
| Total formula weight | 5730.67 |
| Authors | Do, N.Q.,Phan, A.T. (deposition date: 2011-10-07, release date: 2012-10-10, Last modification date: 2024-05-15) |
| Primary citation | Do, N.Q.,Phan, A.T. Monomer-Dimer Equilibrium for the 5'-5' Stacking of Propeller-Type Parallel-Stranded G-Quadruplexes: NMR Structural Study Chemistry, 2012 Cited by PubMed Abstract: Guanine-rich sequence motifs, which contain tracts of three consecutive guanines connected by single non-guanine nucleotides, are abundant in the human genome and can form a robust G-quadruplex structure with high stability. Herein, by using NMR spectroscopy, we investigate the equilibrium between monomeric and 5'-5' stacked dimeric propeller-type G-quadruplexes that are formed by DNA sequences containing GGGT motifs. We show that the monomer-dimer equilibrium depends on a number of parameters, including the DNA concentration, DNA flanking sequences, the concentration and type of cations, and the temperature. We report on the high-definition structure of a simple monomeric G-quadruplex containing three single-residue loops, which could serve as a reference for propeller-type G-quadruplex structures in solution. PubMed: 23019076DOI: 10.1002/chem.201103295 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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