2LE6

Structure of a dimeric all-parallel-stranded G-quadruplex stacked via the 5'-to-5' interface

Summary for 2LE6

• Entry DOI: 10.2210/pdb2le6/pdb
• Related: 1Y8D
• NMR Information: BMRB: 17697
• Descriptor: DNA (5'-D(*GP*IP*GP*TP*GP*GP*GP*TP*GP*GP*GP*TP*GP*GP*GP*T)-3') (1 entity in total)
• Functional Keywords: hiv-1 integrase inhibition, g-quadruplex, anticancer, stacking, dna
• Total number of polymer chains: 2
• Total formula weight: 10214.54

Authors

Do, N.Q.,Lim, K.W.,Teo, M.H.,Heddi, B.,Phan, A.T. (deposition date: 2011-06-10, release date: 2011-08-31, Last modification date: 2024-05-15)

Primary citation

Do, N.Q.,Lim, K.W.,Teo, M.H.,Heddi, B.,Phan, A.T.
Stacking of G-quadruplexes: NMR structure of a G-rich oligonucleotide with potential anti-HIV and anticancer activity
Nucleic Acids Res., 2011

PubMed Abstract: G-rich oligonucleotides T30695 (or T30923), with the sequence of (GGGT)(4), and T40214, with the sequence of (GGGC)(4), have been reported to exhibit anti-HIV and anticancer activity. Here we report on the structure of a dimeric G-quadruplex adopted by a derivative of these sequences in K(+) solution. It comprises two identical propeller-type parallel-stranded G-quadruplex subunits each containing three G-tetrad layers that are stacked via the 5'-5' interface. We demonstrated control over the stacking of the two monomeric subunits by sequence modifications. Our analysis of possible structures at the stacking interface provides a general principle for stacking of G-quadruplexes, which could have implications for the assembly and recognition of higher-order G-quadruplex structures.

PubMed: 21840903
DOI: 10.1093/nar/gkr539

Experimental method

SOLUTION NMR