2LE0

PARP BRCT Domain

Summary for 2LE0

• Entry DOI: 10.2210/pdb2le0/pdb
• NMR Information: BMRB: 17687
• Descriptor: Poly [ADP-ribose] polymerase 1 (1 entity in total)
• Functional Keywords: transferase
• Biological source: Rattus norvegicus (rat)
• Cellular location: Nucleus (By similarity): P27008
• Total number of polymer chains: 1
• Total formula weight: 11672.60

Authors

Mueller, G.,Loeffler, P.,Cuneo, M.,Derose, E.,London, R. (deposition date: 2011-06-03, release date: 2011-10-19, Last modification date: 2024-05-15)

Primary citation

Loeffler, P.A.,Cuneo, M.J.,Mueller, G.A.,Derose, E.F.,Gabel, S.A.,London, R.E.
Structural studies of the PARP-1 BRCT domain.
Bmc Struct.Biol., 11:37-37, 2011

PubMed Abstract: Poly(ADP-ribose) polymerase-1 (PARP-1) is one of the first proteins localized to foci of DNA damage. Upon activation by encountering nicked DNA, the PARP-1 mediated trans-poly(ADP-ribosyl)ation of DNA binding proteins occurs, facilitating access and accumulation of DNA repair factors. PARP-1 also auto-(ADP-ribosyl)ates its central BRCT-containing domain forming part of an interaction site for the DNA repair scaffolding protein X-ray cross complementing group 1 protein (XRCC1). The co-localization of XRCC1, as well as bound DNA repair factors, to sites of DNA damage is important for cell survival and genomic integrity.

PubMed: 21967661
DOI: 10.1186/1472-6807-11-37

Experimental method

SOLUTION NMR