2L9S

Solution structure of Pf1 SID1-mSin3A PAH2 Complex

Summary for 2L9S

• Entry DOI: 10.2210/pdb2l9s/pdb
• Related: 1S5Q 1S5R
• NMR Information: BMRB: 17485
• Descriptor: PHD finger protein 12, Paired amphipathic helix protein Sin3a (2 entities in total)
• Functional Keywords: protein-peptide complex, amphipathic helix motif, transcription
• Biological source: Homo sapiens (human); More
• Cellular location: Nucleus: Q96QT6 Q60520
• Total number of polymer chains: 2
• Total formula weight: 15810.48

Authors

Senthil Kumar, G.,Xie, T.,Zhang, Y.,Radhakrishnan, I. (deposition date: 2011-02-23, release date: 2011-05-11, Last modification date: 2024-05-15)

Primary citation

Kumar, G.S.,Xie, T.,Zhang, Y.,Radhakrishnan, I.
Solution Structure of the mSin3A PAH2-Pf1 SID1 Complex: A Mad1/Mxd1-Like Interaction Disrupted by MRG15 in the Rpd3S/Sin3S Complex.
J.Mol.Biol., 408:987-1000, 2011

PubMed Abstract: Histone deacetylation constitutes an important mechanism for silencing genes. The histone-deacetylase-associated mammalian Rpd3S/Sin3S corepressor complex plays key roles in repressing aberrant gene transcription from cryptic transcription initiation sites and in mitigating RNA polymerase II progression in intragenic regions of actively transcribed genes. The Sin3 corepressor functions as a molecular adaptor linking histone deacetylases on the one hand, with the chromatin targeting subunits Pf1 and MRG15 on the other. Pf1 also functions as an adaptor by interacting with MRG15 and engaging in multivalent interactions with Sin3 targeting among other domains the two N-terminal paired amphipathic helix (PAH) domains that serve as sites of interaction with sequence-specific DNA-binding transcription factors. Here, we structurally and functionally evaluate the interaction between the PAH2 domain of mSin3A and the Sin3 interaction domain 1 (SID1) motif of Pf1 and find the structural aspects to be reminiscent of the interaction between the Mad1/Mxd1 transcription factor and Sin3. Pf1 residues within a highly conserved sequence motif immediately C-terminal to SID1 appear not to be important for the interaction with Sin3 PAH2. Unexpectedly, the MRG15 subunit competes, rather than collaborates, with Sin3 for the Pf1 segment encompassing the two conserved motifs, implying competition between two subunits for another subunit of the same chromatin-modifying complex.

PubMed: 21440557
DOI: 10.1016/j.jmb.2011.03.043

Experimental method

SOLUTION NMR