2L7T

Solution structure of the MFS-bound Sans CEN2 peptide

Summary for 2L7T

• Entry DOI: 10.2210/pdb2l7t/pdb
• Related: 3PVL
• NMR Information: BMRB: 17377
• Descriptor: MFS-bound Sans CEN2 peptide (1 entity in total)
• Functional Keywords: protein peptide, unknown function
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 1
• Total formula weight: 1315.38

Authors

Pan, L.,Wu, L.,Wei, Z.,Zhang, M. (deposition date: 2010-12-22, release date: 2011-03-02, Last modification date: 2024-05-15)

Primary citation

Wu, L.,Pan, L.,Wei, Z.,Zhang, M.
Structure of MyTH4-FERM domains in myosin VIIa tail bound to cargo.
Science, 331:757-760, 2011

PubMed Abstract: The unconventional myosin VIIa (MYO7A) is one of the five proteins that form a network of complexes involved in formation of stereocilia. Defects in these proteins cause syndromic deaf-blindness in humans [Usher syndrome I (USH1)]. Many disease-causing mutations occur in myosin tail homology 4-protein 4.1, ezrin, radixin, moesin (MyTH4-FERM) domains in the myosin tail that binds to another USH1 protein, Sans. We report the crystal structure of MYO7A MyTH4-FERM domains in complex with the central domain (CEN) of Sans at 2.8 angstrom resolution. The MyTH4 and FERM domains form an integral structural and functional supramodule binding to two highly conserved segments (CEN1 and 2) of Sans. The MyTH4-FERM/CEN complex structure provides mechanistic explanations for known deafness-causing mutations in MYO7A MyTH4-FERM. The structure will also facilitate mechanistic and functional studies of MyTH4-FERM domains in other myosins.

PubMed: 21311020
DOI: 10.1126/science.1198848

Experimental method

SOLUTION NMR