2L3C

Solution structure of ADAR2 dsRBM1 bound to LSL RNA

Summary for 2L3C

• Entry DOI: 10.2210/pdb2l3c/pdb
• Related: 2L3J
• Descriptor: Double-stranded RNA-specific editase 1, RNA (34-MER) (2 entities in total)
• Functional Keywords: editing, dsrna recognition, dsrbm, hydrolase-rna complex, hydrolase/rna
• Biological source: Rattus norvegicus (brown rat,rat,rats); More
• Total number of polymer chains: 2
• Total formula weight: 18899.87

Authors

Allain, F.,Stefl, R.,Oberstrass, F. (deposition date: 2010-09-12, release date: 2010-10-27, Last modification date: 2024-05-01)

Primary citation

Stefl, R.,Oberstrass, F.C.,Hood, J.L.,Jourdan, M.,Zimmermann, M.,Skrisovska, L.,Maris, C.,Peng, L.,Hofr, C.,Emeson, R.B.,Allain, F.H.
The Solution Structure of the ADAR2 dsRBM-RNA Complex Reveals a Sequence-Specific Readout of the Minor Groove.
Cell(Cambridge,Mass.), 143:225-237, 2010

PubMed Abstract: Sequence-dependent recognition of dsDNA-binding proteins is well understood, yet sequence-specific recognition of dsRNA by proteins remains largely unknown, despite their importance in RNA maturation pathways. Adenosine deaminases that act on RNA (ADARs) recode genomic information by the site-selective deamination of adenosine. Here, we report the solution structure of the ADAR2 double-stranded RNA-binding motifs (dsRBMs) bound to a stem-loop pre-mRNA encoding the R/G editing site of GluR-2. The structure provides a molecular basis for how dsRBMs recognize the shape, and also more surprisingly, the sequence of the dsRNA. The unexpected direct readout of the RNA primary sequence by dsRBMs is achieved via the minor groove of the dsRNA and this recognition is critical for both editing and binding affinity at the R/G site of GluR-2. More generally, our findings suggest a solution to the sequence-specific paradox faced by many dsRBM-containing proteins that are involved in post-transcriptional regulation of gene expression.

PubMed: 20946981
DOI: 10.1016/j.cell.2010.09.026

Experimental method

SOLUTION NMR