2L1O

Zinc to cadmium replacement in the A. thaliana SUPERMAN Cys2His2 zinc finger induces structural rearrangements of typical DNA base determinant positions

Summary for 2L1O

• Entry DOI: 10.2210/pdb2l1o/pdb
• NMR Information: BMRB: 17091
• Descriptor: Transcriptional regulator SUPERMAN, CADMIUM ION (2 entities in total)
• Functional Keywords: zinc finger domain, cadmium, superman protein, metal binding protein
• Biological source: Arabidopsis thaliana (mouse-ear cress,thale-cress)
• Cellular location: Nucleus: Q38895
• Total number of polymer chains: 1
• Total formula weight: 4607.65

Authors

Malgieri, G.,Zaccaro, L.,Leone, M.,Bucci, E.,Esposito, S.,Baglivo, I.,Del Gatto, A.,Scandurra, R.,Pedone, P.V.,Fattorusso, R.,Isernia, C. (deposition date: 2010-07-31, release date: 2011-06-08, Last modification date: 2024-11-06)

Primary citation

Malgieri, G.,Zaccaro, L.,Leone, M.,Bucci, E.,Esposito, S.,Baglivo, I.,Del Gatto, A.,Russo, L.,Scandurra, R.,Pedone, P.V.,Fattorusso, R.,Isernia, C.
Zinc to cadmium replacement in the A. thaliana SUPERMAN Cys(2) His(2) zinc finger induces structural rearrangements of typical DNA base determinant positions.
Biopolymers, 95:801-810, 2011

PubMed Abstract: Among heavy metals, whose toxicity cause a steadily increasing of environmental pollution, cadmium is of special concern due to its relatively high mobility in soils and potential toxicity at low concentrations. Given their ubiquitous role, zinc fingers domains have been proposed as mediators for the toxic and carcinogenic effects exerted by xenobiotic metals. To verify the structural effects of zinc replacement by cadmium in zinc fingers, we have determined the high resolution structure of the single Cys₂ His₂ zinc finger of the Arabidopsis thaliana SUPERMAN protein (SUP37) complexed to the cadmium ion by means of UV-vis and NMR techniques. SUP37 is able to bind Cd(II), though with a dissociation constant higher than that measured for Zn(II). Cd-SUP37 retains the ββα fold but experiences a global structural rearrangement affecting both the relative orientation of the secondary structure elements and the position of side chains involved in DNA recognition: among them Ser17 side chain, which we show to be essential for DNA binding, experiences the largest displacement.

PubMed: 21618209
DOI: 10.1002/bip.21680

Experimental method

SOLUTION NMR