2KX7

Solution structure of the E.coli RcsD-ABL domain (residues 688-795)

Summary for 2KX7

• Entry DOI: 10.2210/pdb2kx7/pdb
• Related: 1SR2
• Descriptor: Sensor-like histidine kinase yojN (1 entity in total)
• Functional Keywords: alpha-beta-loop (abl) domain, phosphotransfer, rcs regulation, rcsd, two-component system, protein binding
• Biological source: Escherichia coli
• Cellular location: Cell inner membrane; Multi-pass membrane protein (Probable): P39838
• Total number of polymer chains: 1
• Total formula weight: 13168.62

Authors

Rogov, V.V.,Schmoee, K.,Rogova, N.Y.,Loehr, F.,Bernhard, F.,Doetsch, V. (deposition date: 2010-04-27, release date: 2011-04-06, Last modification date: 2024-05-01)

Primary citation

Schmoe, K.,Rogov, V.V.,Rogova, N.Y.,Lohr, F.,Guntert, P.,Bernhard, F.,Dotsch, V.
Structural Insights into Rcs Phosphotransfer: The Newly Identified RcsD-ABL Domain Enhances Interaction with the Response Regulator RcsB.
Structure, 19:577-587, 2011

PubMed Abstract: The Rcs-signaling system is one of the most remarkable phosphorelay pathways in Enterobacteriaceae, comprising several membrane-bound and soluble proteins. Within the complex phosphotransfer pathway, the histidine phosphotransferase (HPt) domain of the RcsD membrane-bound component serves as a crucial factor in modulating the phosphorylation state of the transcription factor RcsB. We have identified a new domain, RcsD-ABL, located N terminally to RcsD-HPt that interacts with RcsB as well. We have determined its structure, characterized its interaction interface with RcsB, and built a structural model of the complex of the RcsD-ABL domain with RcsB. Our results indicate that the effector domain of RcsB, which normally binds to DNA, is recognized by RcsD-ABL, whereas the HPt domain interacts with the phosphoreceiver domain of RcsB.

PubMed: 21481780
DOI: 10.1016/j.str.2011.01.012

Experimental method

SOLUTION NMR