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2KWX

The V27A mutant of influenza A M2 proton channel

2KWX の概要
エントリーDOI10.2210/pdb2kwx/pdb
関連するPDBエントリー2KIH 2RLF
分子名称Matrix protein 2 (1 entity in total)
機能のキーワードm2 proton channel, influenza a, v27a resistant mutant, transport protein
由来する生物種Influenza A virus
細胞内の位置Virion membrane (By similarity): P63231
タンパク質・核酸の鎖数4
化学式量合計17681.17
構造登録者
Pielak, R.M.,Chou, J.J. (登録日: 2010-04-20, 公開日: 2010-09-29, 最終更新日: 2024-05-22)
主引用文献Pielak, R.M.,Chou, J.J.
Solution NMR structure of the V27A drug resistant mutant of influenza A M2 channel.
Biochem.Biophys.Res.Commun., 401:58-63, 2010
Cited by
PubMed Abstract: The M2 protein of influenza A virus forms a proton-selective channel that is required for viral replication. It is the target of the anti-influenza drugs, amantadine and rimantadine. Widespread drug resistant mutants, however, has greatly compromised the effectiveness of these drugs. Here, we report the solution NMR structure of the highly pathogenic, drug resistant mutant V27A. The structure reveals subtle structural differences from wildtype that maybe linked to drug resistance. The V27A mutation significantly decreases hydrophobic packing between the N-terminal ends of the transmembrane helices, which explains the looser, more dynamic tetrameric assembly. The weakened channel assembly can resist drug binding either by destabilizing the rimantadine-binding pocket at Asp44, in the case of the allosteric inhibition model, or by reducing hydrophobic contacts with amantadine in the pore, in the case of the pore-blocking model. Moreover, the V27A structure shows a substantially increased channel opening at the N-terminal end, which may explain the faster proton conduction observed for this mutant. Furthermore, due to the high quality NMR data recorded for the V27A mutant, we were able to determine the structured region connecting the channel domain to the C-terminal amphipathic helices that was not determined in the wildtype structure. The new structural data show that the amphipathic helices are packed much more closely to the channel domain and provide new insights into the proton transfer pathway.
PubMed: 20833142
DOI: 10.1016/j.bbrc.2010.09.008
主引用文献が同じPDBエントリー
実験手法
SOLUTION NMR
構造検証レポート
Validation report summary of 2kwx
検証レポート(詳細版)ダウンロードをダウンロード

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件を2025-04-02に公開中

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