2KSY
Solution nmr structure of sensory rhodopsin II
Summary for 2KSY
| Entry DOI | 10.2210/pdb2ksy/pdb |
| NMR Information | BMRB: 16678 |
| Descriptor | Sensory rhodopsin II, RETINAL (2 entities in total) |
| Functional Keywords | membrane proteins, nmr spectroscopy, receptors, structure elucidation, transmembrane, membrane protein |
| Biological source | Natronomonas pharaonis |
| Total number of polymer chains | 1 |
| Total formula weight | 26740.41 |
| Authors | Gautier, A.,Mott, H.R.,Bostock, M.J.,Kirkpatrick, J.P.,Nietlispach, D. (deposition date: 2010-01-14, release date: 2010-06-02, Last modification date: 2024-11-06) |
| Primary citation | Gautier, A.,Mott, H.R.,Bostock, M.J.,Kirkpatrick, J.P.,Nietlispach, D. Structure determination of the seven-helix transmembrane receptor sensory rhodopsin II by solution NMR spectroscopy. Nat.Struct.Mol.Biol., 17:768-774, 2010 Cited by PubMed Abstract: Seven-helix membrane proteins represent a challenge for structural biology. Here we report the first NMR structure determination of a detergent-solubilized seven-helix transmembrane (7TM) protein, the phototaxis receptor sensory rhodopsin II (pSRII) from Natronomonas pharaonis, as a proof of principle. The overall quality of the structure ensemble is good (backbone r.m.s. deviation of 0.48 A) and agrees well with previously determined X-ray structures. Furthermore, measurements in more native-like small phospholipid bicelles indicate that the protein structure is the same as in detergent micelles, suggesting that environment-specific effects are minimal when using mild detergents. We use our case study as a platform to discuss the feasibility of similar solution NMR studies for other 7TM proteins, including members of the family of G protein-coupled receptors. PubMed: 20512150DOI: 10.1038/nsmb.1807 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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