2KSN

Solution Structure of the N-terminal Domain of DC-UbP/UBTD2

Summary for 2KSN

• Entry DOI: 10.2210/pdb2ksn/pdb
• Descriptor: Ubiquitin domain-containing protein 2 (1 entity in total)
• Functional Keywords: ubtd2, dc-ubp, ubiquitin, signaling protein
• Biological source: Homo sapiens (human)
• Cellular location: Cytoplasm: Q8WUN7
• Total number of polymer chains: 1
• Total formula weight: 15593.36

Authors

Song, A.,Zhou, C.,Guan, X.,Sze, K.,Hu, H. (deposition date: 2010-01-08, release date: 2010-05-19, Last modification date: 2024-05-29)

Primary citation

Song, A.,Zhou, C.,Guan, X.,Sze, K.,Hu, H.
Solution structure of the N-terminal domain of DC-UbP/UBTD2 and its interaction with ubiquitin
Protein Sci., 19:1104-1109, 2010

PubMed Abstract: DC-UbP/UBTD2 is a ubiquitin (Ub) domain-containing protein first identified from dendritic cells, and is implicated in ubiquitination pathway. The solution structure and backbone dynamics of the C-terminal Ub-like (UbL) domain were elucidated in our previous work. To further understand the biological function of DC-UbP, we then solved the solution structure of the N-terminal domain of DC-UbP (DC-UbP_N) and studied its Ub binding properties by NMR techniques. The results show that DC-UbP_N holds a novel structural fold and acts as a Ub-binding domain (UBD) but with low affinity. This implies that the DC-UbP protein, composing of a combination of both UbL and UBD domains, might play an important role in regulating protein ubiquitination and delivery of ubiquitinated substrates in eukaryotic cells.

PubMed: 20440844
DOI: 10.1002/pro.386

Experimental method

SOLUTION NMR