2KQD

First PBZ domain of human APLF protein in complex with ribofuranosyladenosine

2KQD の概要

• エントリーDOI: 10.2210/pdb2kqd/pdb
• 関連するPDBエントリー: 2KQB 2KQC 2KQE
• NMR情報: BMRB: 16596
• 分子名称: Aprataxin and PNK-like factor, ZINC ION, ADENOSINE, ... (4 entities in total)
• 機能のキーワード: adp-ribosylation, dna damage, dna repair, metal-binding, nucleotide-binding, nucleus, zinc, zinc-finger, lyase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10535.84

構造登録者

Neuhaus, D.,Eustermann, S.,Brockmann, C.,Yang, J. (登録日: 2009-11-04, 公開日: 2010-01-19, 最終更新日: 2024-05-01)

主引用文献

Eustermann, S.,Brockmann, C.,Mehrotra, P.V.,Yang, J.C.,Loakes, D.,West, S.C.,Ahel, I.,Neuhaus, D.
Solution structures of the two PBZ domains from human APLF and their interaction with poly(ADP-ribose).
Nat.Struct.Mol.Biol., 17:241-243, 2010

PubMed Abstract: Addition of poly(ADP-ribose) (PAR) is an important post-translational modification in higher eukaryotes. Several DNA repair and checkpoint proteins possess specific PAR-binding zinc-finger (PBZ) modules critical for function. Here, we present solution structures of the two PBZ modules of aprataxin and PNK-like factor (APLF), revealing a novel type of zinc finger. By combining in vivo PAR-binding data with NMR interaction data using PAR fragments, we propose a structural basis for PBZ-PAR recognition.

PubMed: 20098424
DOI: 10.1038/nsmb.1747

実験手法

SOLUTION NMR