2KJI
A divergent ins protein in c. elegans structurally resemble insulin and activates the human insulin receptor
Summary for 2KJI
| Entry DOI | 10.2210/pdb2kji/pdb |
| Related | 4INS |
| Descriptor | Probable insulin-like peptide beta-type 5 (1 entity in total) |
| Functional Keywords | hormone, insulin like peptide, c. elegans, folding, ins-6, cleavage on pair of basic residues, disulfide bond, secreted |
| Biological source | Caenorhabditis elegans (nematode) |
| Total number of polymer chains | 1 |
| Total formula weight | 5304.07 |
| Authors | Hua, Q.X.,Nakarawa, S.H.,Wilken, R.,Ramos, R.R.,Jia, W.H.,Bass, J.,Weiss, M.A. (deposition date: 2009-05-28, release date: 2009-06-16, Last modification date: 2024-10-16) |
| Primary citation | Hua, Q.X.,Nakagawa, S.H.,Wilken, J.,Ramos, R.R.,Jia, W.,Bass, J.,Weiss, M.A. A divergent INS protein in Caenorhabditis elegans structurally resembles human insulin and activates the human insulin receptor. Genes Dev., 17:826-831, 2003 Cited by PubMed Abstract: Caenorhabditis elegans contains a family of putative insulin-like genes proposed to regulate dauer arrest and senescence. These sequences often lack characteristic sequence features of human insulin essential for its folding, structure, and function. Here, we describe the structure and receptor-binding properties of INS-6, a single-chain polypeptide expressed in specific neurons. Despite multiple nonconservative changes in sequence, INS-6 recapitulates an insulin-like fold. Although lacking classical receptor-binding determinants, INS-6 binds to and activates the human insulin receptor. Its activity is greater than that of an analogous single-chain human insulin analog. PubMed: 12654724DOI: 10.1101/gad.1058003 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
Download full validation report
