2KJI

A divergent ins protein in c. elegans structurally resemble insulin and activates the human insulin receptor

2KJI の概要

• エントリーDOI: 10.2210/pdb2kji/pdb
• 関連するPDBエントリー: 4INS
• 分子名称: Probable insulin-like peptide beta-type 5 (1 entity in total)
• 機能のキーワード: hormone, insulin like peptide, c. elegans, folding, ins-6, cleavage on pair of basic residues, disulfide bond, secreted
• 由来する生物種: Caenorhabditis elegans (nematode)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5304.07

構造登録者

Hua, Q.X.,Nakarawa, S.H.,Wilken, R.,Ramos, R.R.,Jia, W.H.,Bass, J.,Weiss, M.A. (登録日: 2009-05-28, 公開日: 2009-06-16, 最終更新日: 2024-10-16)

主引用文献

Hua, Q.X.,Nakagawa, S.H.,Wilken, J.,Ramos, R.R.,Jia, W.,Bass, J.,Weiss, M.A.
A divergent INS protein in Caenorhabditis elegans structurally resembles human insulin and activates the human insulin receptor.
Genes Dev., 17:826-831, 2003

PubMed Abstract: Caenorhabditis elegans contains a family of putative insulin-like genes proposed to regulate dauer arrest and senescence. These sequences often lack characteristic sequence features of human insulin essential for its folding, structure, and function. Here, we describe the structure and receptor-binding properties of INS-6, a single-chain polypeptide expressed in specific neurons. Despite multiple nonconservative changes in sequence, INS-6 recapitulates an insulin-like fold. Although lacking classical receptor-binding determinants, INS-6 binds to and activates the human insulin receptor. Its activity is greater than that of an analogous single-chain human insulin analog.

PubMed: 12654724
DOI: 10.1101/gad.1058003

実験手法

SOLUTION NMR