2KHX

Drosha double-stranded RNA binding motif

Summary for 2KHX

• Entry DOI: 10.2210/pdb2khx/pdb
• NMR Information: BMRB: 16256
• Descriptor: Ribonuclease 3 (1 entity in total)
• Functional Keywords: drosha, rna binding domain, hydrolase, gene regulation, nuclear protein
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 1
• Total formula weight: 9589.89

Authors

Mueller, G.A.,Miller, M.,Ghosh, M.,DeRose, E.F.,London, R.E.,Hall, T. (deposition date: 2009-04-13, release date: 2010-02-23, Last modification date: 2024-05-01)

Primary citation

Mueller, G.A.,Miller, M.T.,Derose, E.F.,Ghosh, M.,London, R.E.,Hall, T.M.
Solution structure of the Drosha double-stranded RNA-binding domain.
Silence, 1:2-2, 2010

PubMed Abstract: Drosha is a nuclear RNase III enzyme that initiates processing of regulatory microRNA. Together with partner protein DiGeorge syndrome critical region 8 (DGCR8), it forms the Microprocessor complex, which cleaves precursor transcripts called primary microRNA to produce hairpin precursor microRNA. In addition to two RNase III catalytic domains, Drosha contains a C-terminal double-stranded RNA-binding domain (dsRBD). To gain insight into the function of this domain, we determined the nuclear magnetic resonance (NMR) solution structure.

PubMed: 20226070
DOI: 10.1186/1758-907X-1-2

Experimental method

SOLUTION NMR