Refined Solution Structure of a Dimer of LAC repressor DNA-Binding domain complexed to its natural operator O1

Summary for 2KEI

Related1L1M 2KEK 2KEJ
DescriptorLactose operon repressor, DNA (5'-D(*GP*AP*AP*TP*TP*GP*TP*GP*AP*GP*CP*GP*GP*AP*TP*AP*AP*CP*AP*AP*TP*TP*T)-3'), DNA (5'-D(P*AP*AP*AP*TP*TP*GP*TP*TP*AP*TP*CP*CP*GP*CP*TP*CP*AP*CP*AP*AP*TP*TP*C)-3') (3 entities in total)
Functional Keywordslac repressor, lac operators, protein-dna complex, dna-binding, repressor, transcription, transcription regulation, transcription-dna complex, transcription/dna
Biological sourceEscherichia coli
Total number of polymer chains4
Total molecular weight27763.69
Romanuka, J.,Folkers, G.,Biris, N.,Tishchenko, E.,Wienk, H.,Kaptein, R.,Boelens, R. (deposition date: 2009-01-30, release date: 2009-05-19, Last modification date: 2011-07-13)
Primary citation
Romanuka, J.,Folkers, G.E.,Biris, N.,Tishchenko, E.,Wienk, H.,Bonvin, A.M.,Kaptein, R.,Boelens, R.
Specificity and affinity of Lac repressor for the auxiliary operators O2 and O3 are explained by the structures of their protein-DNA complexes.
J.Mol.Biol., 390:478-489, 2009
PubMed: 19450607 (PDB entries with the same primary citation)
DOI: 10.1016/j.jmb.2009.05.022
MImport into Mendeley
Experimental method
NMR Information

Structure validation

ClashscoreRamachandran outliersSidechain outliers132.5%13.2%MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all NMR structures

More Asymmetric unit images

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