2KE8
NMR solution structure of metal-modified DNA
Summary for 2KE8
| Entry DOI | 10.2210/pdb2ke8/pdb |
| Related | 2K67 2K68 2K69 |
| Descriptor | DNA (5'-D(*TP*TP*AP*AP*TP*TP*TP*(D33)P*(D33)P*(D33)P*AP*AP*AP*TP*TP*AP*A)-3'), SILVER ION (2 entities in total) |
| Functional Keywords | dna, artificial nucleobase, imidazole nucleoside, ag+ |
| Total number of polymer chains | 2 |
| Total formula weight | 10354.23 |
| Authors | Johannsen, S.,Duepre, N.,Boehme, D.,Mueller, J.,Sigel, R.K.O. (deposition date: 2009-01-27, release date: 2010-02-09, Last modification date: 2024-05-01) |
| Primary citation | Johannsen, S.,Megger, N.,Bohme, D.,Sigel, R.K.,Muller, J. Solution structure of a DNA double helix with consecutive metal-mediated base pairs. Nat.Chem., 2:229-234, 2010 Cited by PubMed Abstract: Metal-mediated base pairs represent a powerful tool for the site-specific functionalization of nucleic acids with metal ions. The development of applications of the metal-modified nucleic acids will depend on the availability of structural information on these double helices. We present here the NMR solution structure of a self-complementary DNA oligonucleotide with three consecutive imidazole nucleotides in its centre. In the absence of transition-metal ions, a hairpin structure is adopted with the artificial nucleotides forming the loop. In the presence of Ag(i) ions, a duplex comprising three imidazole-Ag(+)-imidazole base pairs is formed. Direct proof for the formation of metal-mediated base pairs was obtained from ¹J(¹⁵N,¹⁰⁷/¹⁰⁹Ag) couplings upon incorporation of ¹⁵N-labelled imidazole. The duplex adopts a B-type conformation with only minor deviations in the region of the artificial bases. This work represents the first structural characterization of a metal-modified nucleic acid with a continuous stretch of metal-mediated base pairs. PubMed: 21124482DOI: 10.1038/nchem.512 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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