2K8Y
Solution NMR Structure of Cgi121 from Methanococcus jannaschii. Northeast Structural Genomics Consortium Target MJ0187
Summary for 2K8Y
| Entry DOI | 10.2210/pdb2k8y/pdb |
| Related | 1ZD0 |
| NMR Information | BMRB: 15981 |
| Descriptor | Uncharacterized protein MJ0187 (1 entity in total) |
| Functional Keywords | regulatory subunit of the bud32 kinase, component of the keops complex, structural genomics, psi-2, protein structure initiative, northeast structural genomics consortium, nesg, unknown function |
| Biological source | Methanocaldococcus jannaschii (Methanococcus jannaschii) |
| Total number of polymer chains | 1 |
| Total formula weight | 17092.07 |
| Authors | Rumpel, S.,Fares, C.,Neculai, D.,Arrowsmith, C.,Montelione, G.T.,Sicheri, F.,Northeast Structural Genomics Consortium (NESG) (deposition date: 2008-09-25, release date: 2008-11-04, Last modification date: 2024-05-22) |
| Primary citation | Mao, D.Y.,Neculai, D.,Downey, M.,Orlicky, S.,Haffani, Y.Z.,Ceccarelli, D.F.,Ho, J.S.,Szilard, R.K.,Zhang, W.,Ho, C.S.,Wan, L.,Fares, C.,Rumpel, S.,Kurinov, I.,Arrowsmith, C.H.,Durocher, D.,Sicheri, F. Atomic structure of the KEOPS complex: an ancient protein kinase-containing molecular machine. Mol.Cell, 32:259-275, 2008 Cited by PubMed Abstract: Kae1 is a universally conserved ATPase and part of the essential gene set in bacteria. In archaea and eukaryotes, Kae1 is embedded within the protein kinase-containing KEOPS complex. Mutation of KEOPS subunits in yeast leads to striking telomere and transcription defects, but the exact biochemical function of KEOPS is not known. As a first step to elucidating its function, we solved the atomic structure of archaea-derived KEOPS complexes involving Kae1, Bud32, Pcc1, and Cgi121 subunits. Our studies suggest that Kae1 is regulated at two levels by the primordial protein kinase Bud32, which is itself regulated by Cgi121. Moreover, Pcc1 appears to function as a dimerization module, perhaps suggesting that KEOPS may be a processive molecular machine. Lastly, as Bud32 lacks the conventional substrate-recognition infrastructure of eukaryotic protein kinases including an activation segment, Bud32 may provide a glimpse of the evolutionary history of the protein kinase family. PubMed: 18951093DOI: 10.1016/j.molcel.2008.10.002 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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