2K5U
Solution structure of myirstoylated yeast ARF1 protein, GDP-bound
Summary for 2K5U
| Entry DOI | 10.2210/pdb2k5u/pdb |
| Descriptor | ADP-ribosylation factor 1, GUANOSINE-5'-DIPHOSPHATE (2 entities in total) |
| Functional Keywords | arf, arf1, myristoyl, myrsitoylated, gdp, er-golgi transport, golgi apparatus, gtp-binding, lipoprotein, myristate, nucleotide-binding, protein transport, transport, signaling protein |
| Biological source | Saccharomyces cerevisiae (rat) |
| Cellular location | Golgi apparatus: P11076 |
| Total number of polymer chains | 1 |
| Total formula weight | 21074.80 |
| Authors | Prestegard, J.,Liu, Y. (deposition date: 2008-06-30, release date: 2009-01-27, Last modification date: 2022-03-16) |
| Primary citation | Liu, Y.,Kahn, R.A.,Prestegard, J.H. Structure and Membrane Interaction of Myristoylated ARF1 Structure, 17:79-87, 2009 Cited by PubMed Abstract: ADP-ribosylation factors (ARFs) are small (21 kDa), monomeric GTPases that are important regulators of membrane traffic. When membrane bound, they recruit soluble adaptors to membranes and trigger the assembly of coating complexes involved in cargo selection and vesicular budding. N-myristoylation is a conserved feature of all ARF proteins that is required for its biological functions, although the mechanism(s) by which the myristate acts in ARF functions is not fully understood. Here we present the structure of a myristoylated ARF1 protein, determined by solution NMR methods, and an assessment of the influence of myristoylation on association of ARF1.GDP and ARF1.GTP with lipid bilayers. A model in which myristoylation contributes to both the regulation of guanine nucleotide exchange and stable membrane association is supported. PubMed: 19141284DOI: 10.1016/j.str.2008.10.020 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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