2K42

Solution Structure of the GTPase Binding Domain of WASP in Complex with EspFU, an EHEC Effector

2K42 の概要

• エントリーDOI: 10.2210/pdb2k42/pdb
• NMR情報: BMRB: 16824
• 分子名称: Wiskott-Aldrich syndrome protein, ESPFU (2 entities in total)
• 機能のキーワード: wasp, espfu, gbd, autoinhibition, cytoplasm, cytoskeleton, disease mutation, phosphoprotein, signaling protein
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm, cytoskeleton: P42768
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 12199.59

構造登録者

Cheng, H.-C.,Skehan, B.M.,Campellone, K.G.,Leong, J.M.,Rosen, M.K. (登録日: 2008-05-27, 公開日: 2008-07-22, 最終更新日: 2024-05-29)

主引用文献

Cheng, H.C.,Skehan, B.M.,Campellone, K.G.,Leong, J.M.,Rosen, M.K.
Structural mechanism of WASP activation by the enterohaemorrhagic E. coli effector EspF(U).
Nature, 454:1009-1013, 2008

PubMed Abstract: During infection, enterohaemorrhagic Escherichia coli (EHEC) takes over the actin cytoskeleton of eukaryotic cells by injecting the EspF(U) protein into the host cytoplasm. EspF(U) controls actin by activating members of the Wiskott-Aldrich syndrome protein (WASP) family. Here we show that EspF(U) binds to the autoinhibitory GTPase binding domain (GBD) in WASP proteins and displaces it from the activity-bearing VCA domain (for verprolin homology, central hydrophobic and acidic regions). This interaction potently activates WASP and neural (N)-WASP in vitro and induces localized actin assembly in cells. In the solution structure of the GBD-EspF(U) complex, EspF(U) forms an amphipathic helix that binds the GBD, mimicking interactions of the VCA domain in autoinhibited WASP. Thus, EspF(U) activates WASP by competing directly for the VCA binding site on the GBD. This mechanism is distinct from that used by the eukaryotic activators Cdc42 and SH2 domains, which globally destabilize the GBD fold to release the VCA. Such diversity of mechanism in WASP proteins is distinct from other multimodular systems, and may result from the intrinsically unstructured nature of the isolated GBD and VCA elements. The structural incompatibility of the GBD complexes with EspF(U) and Cdc42/SH2, plus high-affinity EspF(U) binding, enable EHEC to hijack the eukaryotic cytoskeletal machinery effectively.

PubMed: 18650809
DOI: 10.1038/nature07160

実験手法

SOLUTION NMR