2JYM

Solution structure of stem-loop alpha of the hepatitis B virus post-transcriptional regulatory element

Summary for 2JYM

• Entry DOI: 10.2210/pdb2jym/pdb
• NMR Information: BMRB: 15745
• Descriptor: RNA (5'-R(*GP*GP*CP*UP*CP*GP*CP*AP*GP*CP*AP*GP*GP*UP*CP*UP*GP*GP*AP*GP*UP*C)-3') (1 entity in total)
• Functional Keywords: hepatitis b virus, post-transcriptional regulatory element, stem-loop alpha, nmr solution structure, rna
• Total number of polymer chains: 1
• Total formula weight: 7105.27

Authors

Ohlenschlager, O.,Gorlach, M.,Schwalbe, M. (deposition date: 2007-12-14, release date: 2008-04-29, Last modification date: 2024-05-29)

Primary citation

Schwalbe, M.,Ohlenschlager, O.,Marchanka, A.,Ramachandran, R.,Hafner, S.,Heise, T.,Gorlach, M.
Solution structure of stem-loop alpha of the hepatitis B virus post-transcriptional regulatory element
Nucleic Acids Res., 36:1681-1689, 2008

PubMed Abstract: Chronic hepatitis B virus (HBV) infections may lead to severe diseases like liver cirrhosis or hepatocellular carcinoma (HCC). The HBV post-transcriptional regulatory element (HPRE) facilitates the nuclear export of unspliced viral mRNAs, contains a splicing regulatory element and resides in the 3'-region of all viral transcripts. The HPRE consists of three sub-elements alpha (nucleotides 1151-1346), beta1 (nucleotides 1347-1457) and beta2 (nucleotides 1458-1582), which confer together full export competence. Here, we present the NMR solution structure (pdb 2JYM) of the stem-loop alpha (SLalpha, nucleotides 1292-1321) located in the sub-element alpha. The SLalpha contains a CAGGC pentaloop highly conserved in hepatoviruses, which essentially adopts a CUNG-like tetraloop conformation. Furthermore, the SLalpha harbours a single bulged G residue flanked by A-helical regions. The structure is highly suggestive of serving two functions in the context of export of unspliced viral RNA: binding sterile alpha motif (SAM-) domain containing proteins and/or preventing the utilization of a 3'-splice site contained within SLalpha.

PubMed: 18263618
DOI: 10.1093/nar/gkn006

Experimental method

SOLUTION NMR