2JXV
Solution structure of a let-7 miRNA:lin-41 mRNA complex from C. elegans
Summary for 2JXV
| Entry DOI | 10.2210/pdb2jxv/pdb |
| Descriptor | RNA (33-MER) (1 entity in total) |
| Functional Keywords | asymmetric internal loop, stem-loop, gaaa tetraloop, gu mismatch, residual dipolar couplings, rna |
| Total number of polymer chains | 1 |
| Total formula weight | 10625.36 |
| Authors | Cevec, M.,Thibaudeau, C.,Plavec, J. (deposition date: 2007-11-30, release date: 2008-04-29, Last modification date: 2024-05-29) |
| Primary citation | Cevec, M.,Thibaudeau, C.,Plavec, J. Solution structure of a let-7 miRNA:lin-41 mRNA complex from C. elegans. Nucleic Acids Res., 36:2330-2337, 2008 Cited by PubMed Abstract: Let-7 microRNA (miRNA) regulates heterochronic genes in developmental timing of the nematode Caenorhabditis elegans. Binding of miRNA to messenger RNA (mRNA) and structural features of the complex are crucial for gene silencing. We herein present the NMR solution structure of a model mimicking the interaction of let-7 miRNA with its complementary site (LCS 2) in the 3' untranslated region (3'-UTR) of the lin-41 mRNA. A structural study was performed by NMR spectroscopy using NOE restraints, torsion angle restraints and residual dipolar couplings. The 33-nt RNA construct folds into a stem-loop structure that features two stem regions which are separated by an asymmetric internal loop. One of the stems comprises a GU wobble base pair, which does not alter its overall A-form RNA conformation. The asymmetric internal loop adopts a single, well-defined structure in which three uracils form a base triple, while two adenines form a base pair. The 3D structure of the construct gives insight into the structural aspects of interactions between let-7 miRNA and lin-41 mRNA. PubMed: 18296482DOI: 10.1093/nar/gkn088 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
Download full validation report
