2JXJ

NMR structure of the ARID domain from the histone H3K4 demethylase RBP2

Summary for 2JXJ

• Entry DOI: 10.2210/pdb2jxj/pdb
• Descriptor: Histone demethylase JARID1A (1 entity in total)
• Functional Keywords: arid domain, chromatin regulator, developmental protein, dioxygenase, iron, metal-binding, nucleus, oxidoreductase, phosphoprotein, polymorphism, transcription, transcription regulation, zinc, zinc-finger
• Biological source: Homo sapiens (Human)
• Cellular location: Nucleus, nucleolus: P29375
• Total number of polymer chains: 1
• Total formula weight: 10836.68

Authors

Tu, S.,Yuan, C.,Tsai, M. (deposition date: 2007-11-20, release date: 2008-09-30, Last modification date: 2024-05-29)

Primary citation

Tu, S.,Teng, Y.C.,Yuan, C.,Wu, Y.T.,Chan, M.Y.,Cheng, A.N.,Lin, P.H.,Juan, L.J.,Tsai, M.D.
The ARID domain of the H3K4 demethylase RBP2 binds to a DNA CCGCCC motif
Nat.Struct.Mol.Biol., 15:419-421, 2008

PubMed Abstract: The histone H3 lysine 4 demethylase RBP2 contains a DNA binding domain, the AT-rich interaction domain (ARID). We solved the structure of ARID by NMR, identified its DNA binding motif (CCGCCC) and characterized the binding contacts. Immunofluorescence and luciferase assays indicated that ARID is required for RBP2 demethylase activity in cells and that DNA recognition is essential to regulate transcription.

PubMed: 18270511
DOI: 10.1038/nsmb.1400

Experimental method

SOLUTION NMR