2JPZ
Human telomere DNA quadruplex structure in K+ solution hybrid-2 form
Summary for 2JPZ
| Entry DOI | 10.2210/pdb2jpz/pdb |
| Related | 2HY9 2KKA |
| Descriptor | DNA (26-MER) (1 entity in total) |
| Functional Keywords | dna, quadruplex, human telomeric sequence |
| Total number of polymer chains | 1 |
| Total formula weight | 8200.27 |
| Authors | Dai, J.,Carver, M.,Punchihewa, C.,Jones, R.,Yang, D. (deposition date: 2007-05-25, release date: 2007-12-04, Last modification date: 2024-05-01) |
| Primary citation | Dai, J.,Carver, M.,Punchihewa, C.,Jones, R.A.,Yang, D. Structure of the Hybrid-2 type intramolecular human telomeric G-quadruplex in K+ solution: insights into structure polymorphism of the human telomeric sequence Nucleic Acids Res., 35:4927-4940, 2007 Cited by PubMed Abstract: Formation of the G-quadruplex in the human telomeric sequence can inhibit the activity of telomerase, thus the intramolecular telomeric G-quadruplexes have been considered as an attractive anticancer target. Information of intramolecular telomeric G-quadruplex structures formed under physiological conditions is important for structure-based drug design. Here, we report the first structure of the major intramolecular G-quadruplex formed in a native, non-modified human telomeric sequence in K(+) solution. This is a hybrid-type mixed parallel/antiparallel-G-stranded G-quadruplex, one end of which is covered by a novel T:A:T triple capping structure. This structure (Hybrid-2) and the previously reported Hybrid-1 structure differ in their loop arrangements, strand orientations and capping structures. The distinct capping structures appear to be crucial for the favored formation of the specific hybrid-type intramolecular telomeric G-quadruplexes, and may provide specific binding sites for drug targeting. Our study also shows that while the hybrid-type G-quadruplexes appear to be the major conformations in K(+) solution, human telomeric sequences are always in equilibrium between Hybrid-1 and Hybrid-2 structures, which is largely determined by the 3'-flanking sequence. Furthermore, both hybrid-type G-quadruplexes suggest a straightforward means for multimer formation with effective packing in the human telomeric sequence and provide important implications for drug targeting of G-quadruplexes in human telomeres. PubMed: 17626043DOI: 10.1093/nar/gkm522 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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