2JP3

Solution Structure of the human FXYD4 (CHIF) protein in SDS micelles

2JP3 の概要

• エントリーDOI: 10.2210/pdb2jp3/pdb
• 関連するPDBエントリー: 2jol
• 分子名称: FXYD domain-containing ion transport regulator 4 (1 entity in total)
• 機能のキーワード: protein, transcription
• 由来する生物種: Rattus norvegicus (Norway rat)
• 細胞内の位置: Membrane; Single-pass type I membrane protein (Potential): Q63113
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7012.08

構造登録者

Franzin, C.M.,Teriete, P.,Marassi, F.M. (登録日: 2007-04-18, 公開日: 2008-03-04, 最終更新日: 2023-12-20)

主引用文献

Franzin, C.M.,Teriete, P.,Marassi, F.M.
Structural similarity of a membrane protein in micelles and membranes
J.Am.Chem.Soc., 129:8078-8079, 2007

PubMed Abstract: The anisotropic spin interactions measured for membrane proteins in weakly oriented micelles and in oriented lipid bilayers provide independent and potentially complementary high-resolution restraints for structure determination. Here we show that the membrane protein CHIF adopts a similar structure in lipid micelles and bilayers, allowing the restraints from micelle and bilayer samples to be combined in a complementary fashion to enhance the structural information. Back-calculation and assignment of the NMR spectrum of CHIF in oriented lipid bilayers, from the structure determined in micelles, provides additional restraints for structure determination as well as the global orientation of the protein in the membrane. The combined use of solution and solid-state NMR restraints also affords cross-validation for the structural analysis.

PubMed: 17567018
DOI: 10.1021/ja0728371

実験手法

SOLUTION NMR