2JHJ

3-methyladenine dna-glycosylase from Archaeoglobus fulgidus

2JHJ の概要

• エントリーDOI: 10.2210/pdb2jhj/pdb
• 関連するPDBエントリー: 2JHN
• 分子名称: 3-METHYLADENINE DNA-GLYCOSYLASE, SODIUM ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: alka, dna repair, n1-methyladenine, n3-methylcytosine, hyperthermophiles, archaeoglobus fulgidus, hydrolase
• 由来する生物種: ARCHAEOGLOBUS FULGIDUS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 69059.45

構造登録者

Leiros, I.,Nabong, M.P.,Grosvik, K.,Ringvoll, J.,Haugland, G.T.,Uldal, L.,Reite, K.,Olsbu, I.K.,Knaevelsrud, I.,Moe, E.,Andersen, O.A.,Birkeland, N.K.,Ruoff, P.,Klungland, A.,Bjelland, S. (登録日: 2007-02-22, 公開日: 2007-04-10, 最終更新日: 2023-12-13)

主引用文献

Leiros, I.,Nabong, M.P.,Grosvik, K.,Ringvoll, J.,Haugland, G.T.,Uldal, L.,Reite, K.,Olsbu, I.K.,Knaevelsrud, I.,Moe, E.,Andersen, O.A.,Birkeland, N.K.,Ruoff, P.,Klungland, A.,Bjelland, S.
Structural Basis for Enzymatic Excision of N1-Methyladenine and N3-Methylcytosine from DNA
Embo J., 26:2206-, 2007

PubMed Abstract: N(1)-methyladenine (m(1)A) and N(3)-methylcytosine (m(3)C) are major toxic and mutagenic lesions induced by alkylation in single-stranded DNA. In bacteria and mammals, m(1)A and m(3)C were recently shown to be repaired by AlkB-mediated oxidative demethylation, a direct DNA damage reversal mechanism. No AlkB gene homologues have been identified in Archaea. We report that m(1)A and m(3)C are repaired by the AfAlkA base excision repair glycosylase of Archaeoglobus fulgidus, suggesting a different repair mechanism for these lesions in the third domain of life. In addition, AfAlkA was found to effect a robust excision of 1,N(6)-ethenoadenine. We present a high-resolution crystal structure of AfAlkA, which, together with the characterization of several site-directed mutants, forms a molecular rationalization for the newly discovered base excision activity.

PubMed: 17396151
DOI: 10.1038/SJ.EMBOJ.7601662

実験手法

X-RAY DIFFRACTION (1.9 Å)