2J8F

Crystal structure of the modular Cpl-1 endolysin complexed with a peptidoglycan analogue (E94Q mutant in complex with a disaccharide- pentapeptide)

2J8F の概要

• エントリーDOI: 10.2210/pdb2j8f/pdb
• 関連するPDBエントリー: 1H09 1OBA 2IXU 2IXV 2J8G
• 分子名称: LYSOZYME, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-methyl 2-acetamido-3-O-[(1R)-1-carboxyethyl]-2-deoxy-beta-D-glucopyranoside, ALANINE, ... (6 entities in total)
• 機能のキーワード: antimicrobial, muein hydrolase, bacteriolytic enzyme, pneumococcal cell wall degradation, lysozyme, hydrolase, glycosidase, multimodular
• 由来する生物種: BACTERIOPHAGE CP-1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 40612.73

構造登録者

Perez-Dorado, I.,Hermoso, J.A. (登録日: 2006-10-25, 公開日: 2007-07-03, 最終更新日: 2023-12-13)

主引用文献

Perez-Dorado, I.,Campillo, N.E.,Monterroso, B.,Hesek, D.,Lee, M.,Paez, J.A.,Garcia, P.,Martinez-Ripoll, M.,Garcia, J.L.,Mobashery, S.,Menendez, M.,Hermoso, J.A.
Elucidation of the Molecular Recognition of Bacterial Cell Wall by Modular Pneumococcal Phage Endolysin Cpl-1.
J.Biol.Chem., 282:24990-, 2007

PubMed Abstract: Pneumococcal bacteriophage-encoded lysins are modular proteins that have been shown to act as enzymatic antimicrobial agents (enzybiotics) in treatment of streptococcal infections. The first x-ray crystal structures of the Cpl-1 lysin, encoded by the pneumococcal phage Cp-1, in complex with three bacterial cell wall peptidoglycan (PG) analogues are reported herein. The Cpl-1 structure is folded in two well defined modules, one responsible for anchoring to the pneumococcal cell wall and the other, a catalytic module, that hydrolyzes the PG. Conformational rearrangement of Tyr-127 is a critical event in molecular recognition of a stretch of five saccharide rings of the polymeric peptidoglycan (cell wall). The PG is bound at a stretch of the surface that is defined as the peptidoglycan-binding sites 1 and 2, the juncture of which catalysis takes place. The peptidoglycan-binding site 1 binds to a stretch of three saccharides of the peptidoglycan in a conformation essentially identical to that of the peptidoglycan in solution. In contrast, binding of two peptidoglycan saccharides at the peptidoglycan-binding site 2 introduces a kink into the solution structure of the peptidoglycan, en route to catalytic turnover. These findings provide the first structural evidence on recognition of the peptidoglycan and shed light on the discrete events of cell wall degradation by Cpl-1.

PubMed: 17581815
DOI: 10.1074/JBC.M704317200

実験手法

X-RAY DIFFRACTION (1.84 Å)