2J7A

Crystal structure of cytochrome c nitrite reductase NrfHA complex from Desulfovibrio vulgaris

Summary for 2J7A

• Entry DOI: 10.2210/pdb2j7a/pdb
• Descriptor: CYTOCHROME C NITRITE REDUCTASE NRFA, CYTOCHROME C QUINOL DEHYDROGENASE NRFH, HEME C, ... (7 entities in total)
• Functional Keywords: quinol dehydrogenase, cytochrome c nitrite reductase, nrfa, nrfh, napc/nirt family, membrane complex, oxidoreductase
• Biological source: DESULFOVIBRIO VULGARIS; More
• Total number of polymer chains: 18
• Total formula weight: 850740.53

Authors

Rodrigues, M.L.,Oliveira, T.F.,Pereira, I.A.C.,Archer, M. (deposition date: 2006-10-06, release date: 2006-12-06, Last modification date: 2019-11-06)

Primary citation

Rodrigues, M.L.,Oliveira, T.F.,Pereira, I.A.C.,Archer, M.
X-Ray Structure of the Membrane-Bound Cytochrome C Quinol Dehydrogenase Nrfh Reveals Novel Haem Coordination.
Embo J., 25:5951-, 2006

PubMed Abstract: Oxidation of membrane-bound quinol molecules is a central step in the respiratory electron transport chains used by biological cells to generate ATP by oxidative phosphorylation. A novel family of cytochrome c quinol dehydrogenases that play an important role in bacterial respiratory chains was recognised in recent years. Here, we describe the first structure of a cytochrome from this family, NrfH from Desulfovibrio vulgaris, which forms a stable complex with its electron partner, the cytochrome c nitrite reductase NrfA. One NrfH molecule interacts with one NrfA dimer in an asymmetrical manner, forming a large membrane-bound complex with an overall alpha(4)beta(2) quaternary arrangement. The menaquinol-interacting NrfH haem is pentacoordinated, bound by a methionine from the CXXCHXM sequence, with an aspartate residue occupying the distal position. The NrfH haem that transfers electrons to NrfA has a lysine residue from the closest NrfA molecule as distal ligand. A likely menaquinol binding site, containing several conserved and essential residues, is identified.

PubMed: 17139260
DOI: 10.1038/SJ.EMBOJ.7601439

Experimental method

X-RAY DIFFRACTION (2.3 Å)