2J19

Ferrous Chloroperoxidase (high dose data set)

Summary for 2J19

• Entry DOI: 10.2210/pdb2j19/pdb
• Related: 1CPO 2CIV 2CIW 2CIX 2CIY 2CIZ 2CJ0 2CJ1 2CJ2 2CPO 2J18
• Related PRD ID: PRD_900111
• Descriptor: CHLOROPEROXIDASE, alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (10 entities in total)
• Functional Keywords: pyrrolidone carboxylic acid, manganese, peroxidase, glycoprotein, metal-binding, oxidoreductase, iron, heme, chloride
• Biological source: CALDARIOMYCES FUMAGO
• Total number of polymer chains: 1
• Total formula weight: 36853.87

Authors

Beitlich, T.,Kuhnel, K.,Schulze-Briese, C.,Shoeman, R.L.,Schlichting, I. (deposition date: 2006-08-09, release date: 2006-12-18, Last modification date: 2024-10-23)

Primary citation

Beitlich, T.,Kuhnel, K.,Schulze-Briese, C.,Shoeman, R.L.,Schlichting, I.
Cryoradiolytic Reduction of Crystalline Heme Proteins: Analysis by Uv-Vis Spectroscopy and X-Ray Crystallography
J.Synchrotron Radiat., 14:11-, 2007

PubMed Abstract: The X-ray crystallographic analysis of redox-active systems may be complicated by photoreduction. Although radiolytic reduction by the probing X-ray beam may be exploited to generate otherwise short-lived reaction intermediates of metalloproteins, it is generally an undesired feature. Here, the X-ray-induced reduction of the three heme proteins myoglobin, cytochrome P450cam and chloroperoxidase has been followed by on-line UV-Vis absorption spectroscopy. All three systems showed a very rapid reduction of the heme iron. In chloroperoxidase the change of the ionization state from ferric to ferrous heme is associated with a movement of the heme-coordinating water molecule. The influence of the energy of the incident X-ray photons and of the presence of scavengers on the apparent reduction rate of ferric myoglobin crystals was analyzed.

PubMed: 17211068
DOI: 10.1107/S0909049506049806

Experimental method

X-RAY DIFFRACTION (1.75 Å)