2J0Q

The crystal structure of the Exon Junction Complex at 3.2 A resolution

2J0Q の概要

• エントリーDOI: 10.2210/pdb2j0q/pdb
• 関連するPDBエントリー: 1P27 2J0S 2J0U
• 分子名称: ATP-DEPENDENT RNA HELICASE DDX48, PROTEIN MAGO NASHI HOMOLOG, RNA-BINDING PROTEIN 8A, ... (8 entities in total)
• 機能のキーワード: hydrolase, mrna processing, mrna splicing, mrna transport, nuclear protein, dead-box helicase, rna-binding
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• 細胞内の位置: Nucleus: P38919 P61326 Q9Y5S9; Cytoplasm, perinuclear region: O15234
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 198964.38

構造登録者

Bono, F.,Ebert, J.,Lorentzen, E.,Conti, E. (登録日: 2006-08-04, 公開日: 2006-08-30, 最終更新日: 2023-12-13)

主引用文献

Bono, F.,Ebert, J.,Lorentzen, E.,Conti, E.
The Crystal Structure of the Exon Junction Complex Reveals How It Maintains a Stable Grip on Mrna.
Cell(Cambridge,Mass.), 126:713-, 2006

PubMed Abstract: The exon junction complex (EJC) plays a major role in posttranscriptional regulation of mRNA in metazoa. The EJC is deposited onto mRNA during splicing and is transported to the cytoplasm where it influences translation, surveillance, and localization of the spliced mRNA. The complex is formed by the association of four proteins (eIF4AIII, Barentsz [Btz], Mago, and Y14), mRNA, and ATP. The 2.2 A resolution structure of the EJC reveals how it stably locks onto mRNA. The DEAD-box protein eIF4AIII encloses an ATP molecule and provides the binding sites for six ribonucleotides. Btz wraps around eIF4AIII and stacks against the 5' nucleotide. An intertwined network of interactions anchors Mago-Y14 and Btz at the interface between the two domains of eIF4AIII, effectively stabilizing the ATP bound state. Comparison with the structure of the eIF4AIII-Btz subcomplex that we have also determined reveals that large conformational changes are required upon EJC assembly and disassembly.

PubMed: 16923391
DOI: 10.1016/J.CELL.2006.08.006

実験手法

X-RAY DIFFRACTION (3.2 Å)